| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044119 |
| Chromosome: | chromosome 1 |
| Location: | 2631090 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015700 | SMM1 | (1 of 8) 3.4.24.71 - Endothelin-converting enzyme 1 / ECE-1; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACCCCAGACCCACCGGCCCCGGCCCGTCCGCCCCAGCACCCAACCCAC |
| Internal bar code: | AAATCTGGGTTTGGGTGCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1210 |
| LEAP-Seq percent confirming: | 55.7377 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGCTTCAAGTCCATCCA |
| Suggested primer 2: | CTGTAGGCCTGCTTTGGGAA |