| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044125 |
| Chromosome: | chromosome 12 |
| Location: | 4084660 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGGTTGCCGAGCTTGGGGATAGGCAGGGCGACCAGAGGGAGGGCGG |
| Internal bar code: | TCCCCTGAGGCAAGTATCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5113 |
| LEAP-Seq percent confirming: | 25.641 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 117 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGACTGGGAGGCAGATTA |
| Suggested primer 2: | CACAAACAAAGCGTGGGAGG |