Insertion junction: CLIP2.044128_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044128
Chromosome:chromosome 2
Location:3228443
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g097050 (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGGGAGGATCGGAAATCGTCGCTGCGCGCGTGACGGTGCGGTTGTCG
Internal bar code:GCGAAGGTTCGGTTTACCGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:635
LEAP-Seq percent confirming:2.89855
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:67
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTGTGAGCGTCCGTTGTG
Suggested primer 2:CTTGCCCTCGAACCAGTCAT