Insertion junction: CLIP2.044137_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.044137
Chromosome:chromosome 12
Location:3252572
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre12.g499451 (1 of 1) PTHR14089//PTHR14089:SF2 - CELL CYCLE CONTROL PROTEIN // PRE-MRNA-SPLICING FACTOR CWC2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAGCTCAAGGCGGCCCTGAAGAAGGGCACCTCGGTGGGGTCCTACCC
Internal bar code:TGCTTTCACGGGGGGTGAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:727
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGTTTTTCCCCTTGGTG
Suggested primer 2:GGCCCCGTCAACAACATCTA