| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044145 |
| Chromosome: | chromosome 10 |
| Location: | 3896017 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446450 | (1 of 1) PTHR26402:SF316 - TRANSCRIPTIONAL REGULATORY PROTEIN CUSR | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGGTATGACGATCACGCTGGCCCTGCTGTCATGTGCAGCACTGCAT |
| Internal bar code: | GGCGTATCGCGTAATTTCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3830 |
| LEAP-Seq percent confirming: | 64.2857 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACAATCTCCTCCGCACC |
| Suggested primer 2: | TTCCTGACCTCAACTCGTGC |