| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044155 |
| Chromosome: | chromosome 6 |
| Location: | 2925463 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273200 | (1 of 1) K03762 - MFS transporter, MHS family, proline/betaine transporter (proP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTGGCTGCTTGCCCACCTACCAAACGGTGAGTGCTGCAGCCTCGGCT |
| Internal bar code: | AGCCTGGAAAGAGAGGTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2516 |
| LEAP-Seq percent confirming: | 92.1053 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAATCAGGGTGCGGAAG |
| Suggested primer 2: | GGAGTGGTGAGTCTCTCCCT |