Insertion junction: CLIP2.044171_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.044171
Chromosome:chromosome 2
Location:7624425
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147850 CLPY2,HSLU2,HUV2 (1 of 2) K03667 - ATP-dependent HslUV protease ATP-binding subunit HslU (hslU); Peptidase subunit of mitochondrial HslUV protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGAAGCTGCTGAGCAGCGAGGCGGTGACGCGGGAGGCGCTGAGGGCGGC
Internal bar code:TCTGGGGGGAAAATGACGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1088
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GACTTAGGGGGAGTTGTGCC
Suggested primer 2:CCAACACCAACACCAACACC