Insertion junction: CLIP2.044188_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.044188
Chromosome:chromosome 12
Location:3095275
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g501050 ALDH25,ALD6 Aldehyde/histidinol dehydrogenase; (1 of 1) 1.1.1.1//1.2.1.10 - Alcohol dehydrogenase / Aldehyde reductase // Acetaldehyde dehydrogenase (acetylating) / Aldehyde dehydrogenase (acylating) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTGCCGTGTGGGTGGGTGCCGCGGCCGCGACCGCTGCCGCGGCCCGG
Internal bar code:ACGACTTCAATCGGTGTTGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:881
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGAAGGAGTGGGCTCAGC
Suggested primer 2:CATTCCCAACTCCTCCACCC