Insertion junction: CLIP2.044197_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.044197
Chromosome:chromosome 1
Location:6306309
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g044800 DGAT1,PFL1 (1 of 1) 2.3.1.54 - Formate C-acetyltransferase / Pyruvate formate-lyase; Pyruvate-formate lyase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTTGCCTCCTCCTCCTCTGCTCCCTCCCTCTCTCCCTCCGTCCCTCCA
Internal bar code:AATCGATACATATGATAAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:495
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAAGTCCGTCATGAGGCC
Suggested primer 2:GAAGACCGACCTGAAGCACA