Insertion junction: CLIP2.044199_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.044199
Chromosome:chromosome 3
Location:2892374
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g162550 MSN1 methylthioadenosine nucleosidase; (1 of 1) 2.4.2.28//3.2.2.9 - S-methyl-5'-thioadenosine phosphorylase / MTAPase // Adenosylhomocysteine nucleosidase / S-adenosylhomocysteine/5'-methylthioadenosine nucleosidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGTCTCCCCGCCTCCCGGCCCCCTGCATCCCCTCTTCCTCCTGGCCC
Internal bar code:GTCGTTAATCCATCCGGCCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:68
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGAAGAGGCAGGAGAGG
Suggested primer 2:TTTGTCCGTGTAGTCGAGGC