| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044215 |
| Chromosome: | chromosome 6 |
| Location: | 7976095 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304550 | PAN2,PAR1 | (1 of 1) K12571 - PAB-dependent poly(A)-specific ribonuclease subunit 2 (PAN2); Poly(A)-ribonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTGCCAGCCCGTTCCCCTCTTTTCCTCGTTCGCATGAATGGTTATTC |
| Internal bar code: | ATCGTGCGGGTTACTTAGAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 94 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTAGGAGAGGACAGGGGA |
| Suggested primer 2: | TCCTTTGCCGCATCTCACAT |