| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044220 |
| Chromosome: | chromosome 2 |
| Location: | 6005080 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118600 | FTSZ3,FTSZ1 | (1 of 2) 3.6.5.6 - Tubulin GTPase; Plastid division protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTTGCAGGCACGGCCATGCTGGGTGTGGGCGCCGCCTCCACAGCCA |
| Internal bar code: | TACAGTCTGTATTCACTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1685 |
| LEAP-Seq percent confirming: | 84.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACTGCTCGTCCACAACT |
| Suggested primer 2: | GACTCCGTGATCGTCATCCC |