Insertion junction: CLIP2.044220_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.044220
Chromosome:chromosome 2
Location:6005081
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118600 FTSZ3,FTSZ1 (1 of 2) 3.6.5.6 - Tubulin GTPase; Plastid division protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGCGCGCAATGTCAAGCACCGTACAGTAAAACACTAACGCCGTCCAGT
Internal bar code:AAGGAAGGCCGACCAGATTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:5393
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:107

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCCGTGATCGTCATCCC
Suggested primer 2:CGTACTGCTCGTCCACAACT