Insertion junction: CLIP2.044222_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.044222
Chromosome:chromosome 2
Location:5955437
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118050 CYCU1 U-type cyclin; (1 of 1) PTHR15615:SF20 - CYCLIN FAMILY PROTEIN-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAGACCAGGGCCATAACGAAGCACACGGGGCTGCACTTGGTGAACTTG
Internal bar code:GTCCAACGATCGGGCAGTTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:5194
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTTGGGAAGTGAGACGG
Suggested primer 2:AGTGGTCGTCTGTGAGCTTG