Insertion junction: CLIP2.044222_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.044222
Chromosome:chromosome 2
Location:5955438
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118050 CYCU1 U-type cyclin; (1 of 1) PTHR15615:SF20 - CYCLIN FAMILY PROTEIN-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGACCTGCTGGCACAGGTGCGGGACACACGAGGGCGCTCGGGCAGGGG
Internal bar code:GTCCAACGATCGGGCAGTTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:185
LEAP-Seq percent confirming:4.54545
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGGTCGTCTGTGAGCTTG
Suggested primer 2:GGACTTGGGAAGTGAGACGG