Insertion junction: CLIP2.044229_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.044229
Chromosome:chromosome 3
Location:1658563
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g152900 MPA5 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACTTTCTCTGATATTCACAATGTGCGTGTGCATGCGCGCGCAGGCGAG
Internal bar code:TACGTAGACTTGAGCATCCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2898
LEAP-Seq percent confirming:97.7778
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGGCGCGTGCATTACTAC
Suggested primer 2:ACCTTAGTCCCTGATCCCCC