| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044235 |
| Chromosome: | chromosome 12 |
| Location: | 4608700 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCAACATCTTGAGGGTGCCTGGCCGCGCGGGTGCAGGCGGCAGGCAG |
| Internal bar code: | AACGTGCCGCGTGAAGCACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1267 |
| LEAP-Seq percent confirming: | 65.3061 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCTACTACGAGAACGAC |
| Suggested primer 2: | TTGTTTGCGCCATGTTGAGG |