Insertion junction: CLIP2.044247_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044247
Chromosome:chromosome 15
Location:1744341
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre19.g751197 (1 of 1) K14794 - ribosomal RNA-processing protein 12 (RRP12) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACATCCCCGGGCCTTCTGGCGCCACACCAGACTCCTGGACCTCAGCA
Internal bar code:TGATACAGGTGGAGAGACGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:742
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:91
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:GTACACACACACACACACGC
Suggested primer 2:GGCGCAGTACAACTTGCAAA