Insertion junction: CLIP2.044249_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044249
Chromosome:chromosome 2
Location:4742233
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTCCAGGAAGCTGATTTAGCGCATTTGTGGCACCAGACGGTGGACGTG
Internal bar code:TGTTTTAGTGCCTCTGGAAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1956
LEAP-Seq percent confirming:21.4286
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCCTGCAATCAAAGCACA
Suggested primer 2:TTGATTTGCTGTGTGGCTGC