| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044249 |
| Chromosome: | chromosome 16 |
| Location: | 6829709 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672050 | (1 of 10) PF13460 - NAD(P)H-binding (NAD_binding_10) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCCGCCCGCGCCTTGGCGCCACGCTCCAGCATCCCCGGCCTTGAAGC |
| Internal bar code: | GGAGAGCTATGGCTTTTTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAGCGTTGTATCGGAG |
| Suggested primer 2: | GTTGGTCAACACTGCGTGAC |