| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044258 |
| Chromosome: | chromosome 9 |
| Location: | 5270772 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407150 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTGACACGGAGGCAGCACACACACAAAGCGGGGGGTGCAAGGTTACA |
| Internal bar code: | AGATGAATTGTGGCAGACCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 77.4194 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGATGGCCGCAGTACATA |
| Suggested primer 2: | ACCACTGCTACTGCCATCAC |