Insertion junction: CLIP2.044262_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.044262
Chromosome:chromosome 10
Location:502217
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421079 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTACGCTGTTTACCTAAATCGCGACATCACAACCCCCCTGCTGCCCCT
Internal bar code:TATCAGGGTTGCGATGGACACG

Confirmation - LEAP-Seq

LEAP-Seq distance:4078
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:117

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGCCACATGAGGTCGAAG
Suggested primer 2:ATGTTATTCTTGTCGCGCGC