| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044270 |
| Chromosome: | chromosome 16 |
| Location: | 5065501 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685800 | (1 of 4) 3.1.2.2//3.1.2.20 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase // Acyl-CoA hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCTGGAGAAGTCAACAGGCAGAGTCGCGGGCGTACGGCTCAGGGGCT |
| Internal bar code: | CTAGGCTGCAATGCGGTGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCGAAAAACGGCGTGAT |
| Suggested primer 2: | TTAGCAGTAGCAGCACGGAC |