Insertion junction: CLIP2.044288_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.044288
Chromosome:chromosome 12
Location:3137773
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g500700 LCL2 (1 of 125) IPR001841 - Zinc finger, RING-type; Long-chain-fatty-acid-CoA ligase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAATTATGCAAATGGATAAAAGCTCTGGAGATAGGTTTTCGAGGACTT
Internal bar code:CTAATACACACCTTACAGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1056
LEAP-Seq percent confirming:90.4762
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGTTTTTCCGCTGCTT
Suggested primer 2:CCGCGCACGTAACTTATGTG