| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044289 |
| Chromosome: | chromosome 2 |
| Location: | 5930343 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117850 | (1 of 1) K11835 - ubiquitin carboxyl-terminal hydrolase 4/11/15 [EC:3.4.19.12] (USP4_11_15, UBP12) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCCCATACTGAGCGCAACCCATCAGCATCAGGGTCACAGGGTGGGGC |
| Internal bar code: | TTCTTGTTGGACAGCACGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1364 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTGGTAGCCTTTGACCC |
| Suggested primer 2: | CGTGTACCTCTGGATCCACG |