Insertion junction: CLIP2.044291_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044291
Chromosome:chromosome 6
Location:2345604
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g267750 (1 of 1) PF00050//PF00089 - Kazal-type serine protease inhibitor domain (Kazal_1) // Trypsin (Trypsin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACCACACCAAACGGGTTCACGATCTCGCCGTCCAGGGTGCGGTAACG
Internal bar code:ATCTTCCTACAAGGCACCTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:5892
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:76

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCAACGCGTTTTGGAAGT
Suggested primer 2:GTAGAGGTAGCCATCCGTGC