Insertion junction: CLIP2.044291_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.044291
Chromosome:chromosome 6
Location:2345631
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g267750 (1 of 1) PF00050//PF00089 - Kazal-type serine protease inhibitor domain (Kazal_1) // Trypsin (Trypsin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACTCCACCATGTCCACCGCCGACCCCAATGTGTAAGTCACTGTGTACG
Internal bar code:ATCTTCCTACAAGGCACCTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:984
LEAP-Seq percent confirming:21.0526
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGAGGTAGCCATCCGTGC
Suggested primer 2:GAGCAACGCGTTTTGGAAGT