| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044295 |
| Chromosome: | chromosome 3 |
| Location: | 8757676 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207825 | (1 of 1) K03241 - translation initiation factor eIF-2B subunit gamma (EIF2B3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTCCTCCTCCTCCCCTTCCCCTCTCAGGTGGTCATCGGCGAGAAGG |
| Internal bar code: | CTACGTAGGGTTAGCGCTTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCGTAACCCCTTGCGAC |
| Suggested primer 2: | CGCTTACCACAAACACGGTG |