Insertion junction: CLIP2.044298_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.044298
Chromosome:chromosome 13
Location:4017309
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g590550 COA5,COAD1 (1 of 1) 2.7.7.3 - Pantetheine-phosphate adenylyltransferase / PPAT; Pantetheine-phosphate adenylyltransferase, CoA biosynthesis intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAACCATGCCTTGTGCCGCGCACCCAACACCCCCAACGCACACGCCTC
Internal bar code:TAGCCTTGGAACCAAATCCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:362
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGCCTACAACACGCTGTT
Suggested primer 2:GAGGAGTGTGGAGGTGTTGG