| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.044302 |
| Chromosome: | chromosome 12 |
| Location: | 9561271 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539900 | (1 of 1) K00166 - 2-oxoisovalerate dehydrogenase E1 component alpha subunit (BCKDHA, bkdA1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCGGCTCACCATGTCCTGCCCCCCCAGCCCCGCCGCCGAGCCGATGT |
| Internal bar code: | GGGTTTTATCCTCCGTTGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGACCATGGACACCAT |
| Suggested primer 2: | CACCTGGTTAGCGAACTGGT |