Insertion junction: CLIP2.044330_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.044330
Chromosome:chromosome 13
Location:838028
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g567250 (1 of 2) IPR007087//IPR015880 - Zinc finger, C2H2 // Zinc finger, C2H2-like intron
Cre13.g567300 (1 of 1) PTHR18968//PTHR18968:SF86 - THIAMINE PYROPHOSPHATE ENZYMES // SUBFAMILY NOT NAMED 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGCAAGTCCTAGCTTACCATCATACGCGCTGTACTCGCCAGCTGCCTC
Internal bar code:CAAGGATGGCACGCAGCGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1739
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTGTCGAATTCAAGCTC
Suggested primer 2:CACCACCACTCTCATGACCC