| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.044334 |
| Chromosome: | chromosome 9 |
| Location: | 3036239 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393691 | KDG4 | (1 of 1) 2.7.1.138 - Ceramide kinase / Acylsphingosine kinase; Diacylglycerol/ceramide/sphingosine kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCAGCCAGGGGCGCGGGCGCGGCGTGCGTGGCGGCGGTGGCGTGAA |
| Internal bar code: | CCCGATGTTAGTCTGAACAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGATGTCGGGTCATGGG |
| Suggested primer 2: | ACAGCGGGAAGATGAACAGG |