Insertion junction: CLIP2.044336_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.044336
Chromosome:chromosome 17
Location:2771421
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGAGAAGACGTCGCACGCGTCGTTCACGTTGAACTGAGGGAGGGAAA
Internal bar code:GTCTCAACTCATTATACATTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:864
LEAP-Seq percent confirming:23.5294
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TAAAACTGCTAGGCGCACCA
Suggested primer 2:GGAGGAGAAGGAGGAGGAGG