| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044337 |
| Chromosome: | chromosome 3 |
| Location: | 3836331 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170150 | (1 of 2) PTHR10857//PTHR10857:SF0 - COPINE // PROTEIN GEM-4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTCAGCTCCACCCAACCCAGCGGCCGGTGAGACCCGTTTGACTCGTA |
| Internal bar code: | CGCGCGGAAGAGGGACTGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2154 |
| LEAP-Seq percent confirming: | 93.1507 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCGCATACCTGCTACTG |
| Suggested primer 2: | GCAGACAGACCCTCAGACAC |