| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044339 |
| Chromosome: | chromosome 10 |
| Location: | 1199684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426152 | MGL1 | Methionine gamma-lyase; (1 of 1) K01761 - methionine-gamma-lyase (E4.4.1.11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCACTGAGATCCACTACGTCCCTCTGTACGGCTAAGGAAGGAGTAGC |
| Internal bar code: | TTATACGCTAACATCCCTTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2527 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACATGACCGAACCCTTCC |
| Suggested primer 2: | TGAAGAGGAGGATGAGGGGG |