| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044344 |
| Chromosome: | chromosome 16 |
| Location: | 6592938 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673950 | (1 of 1) 2.6.1.80 - Nicotianamine aminotransferase / Nicotianamine transaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGGTGCATGAGGCCAAGGTTTCCTAGCAGCAGCATGCAGCAGTCGG |
| Internal bar code: | TTTGGATAATTTGGTTTAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2882 |
| LEAP-Seq percent confirming: | 95.082 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACCAAGCACGAGTCCAG |
| Suggested primer 2: | TAATGAAGGCCCGGGGTAGA |