Insertion junction: CLIP2.044347_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.044347
Chromosome:chromosome 2
Location:6731481
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g387578 (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGGGGGTTGTTGAACAGCGTAGGAGCGGTGATGGTGGTGGGAAGGAG
Internal bar code:GGTTCTACCACCGCGGTCTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1665
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGCTGCCGCTTTAAGAT
Suggested primer 2:CCCACCAGGACTTACGCAAT