| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.044359 |
| Chromosome: | chromosome 16 |
| Location: | 1197207 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650625 | (1 of 1) PTHR11757:SF3 - OLIGOPEPTIDASE B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACACAACAGACCCAGTCAGAGCTGCTAGAGGCCATGACCGCCCGGGTG |
| Internal bar code: | CGTTGTTTTGGCACCTAGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTACTGCTACTGCTGCT |
| Suggested primer 2: | ACGCGGTCTCCTTTCCAATT |