| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044391 |
| Chromosome: | chromosome 16 |
| Location: | 7544212 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683147 | (1 of 44) IPR000048 - IQ motif, EF-hand binding site | 5'UTR | |
| Cre16.g683259 | MCP29 | (1 of 1) PTHR24089:SF270 - SOLUTE CARRIER FAMILY 25 MEMBER 48; Mitochondrial substrate carrier protein | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTGTCCTGATGCTTTTTATAGGCTTGAGTATGTGACTTTTATCAATT |
| Internal bar code: | ATACGCATACTATTAGATTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1923 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTGACTGTACTGAAGCG |
| Suggested primer 2: | GCTTTGGGGATGTCACGAGA |