| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044394 |
| Chromosome: | chromosome 12 |
| Location: | 7853157 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553700 | PDO2 | Protein disulfide oxidoreductase; (1 of 3) PTHR13887//PTHR13887:SF10 - GLUTATHIONE S-TRANSFERASE KAPPA // FRNE PROTEIN-LIKE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAAGAATGCTGCTTCGGTACTCGCGTCATGGCGGATCCTCGCGTCCG |
| Internal bar code: | TGTGTTACCCGTCCAGTCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACTGCAGCTTGCTCAGG |
| Suggested primer 2: | ATTGTCATCCTTCGGGCGTT |