| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.044411 |
| Chromosome: | chromosome 3 |
| Location: | 6532525 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194100 | MAPKKK3 | (1 of 1) PF14381 - Ethylene-responsive protein kinase Le-CTR1 (EDR1); Mitogen-Activated Protein Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATCTGAACTCTCTGAGCGCCTGCGCCCTCCGTAATGGGACCCAGGGG |
| Internal bar code: | CCGAGGAGTCAGTTCAGCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 958 |
| LEAP-Seq percent confirming: | 65.3846 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAAGCTTTGCTGGCGTTG |
| Suggested primer 2: | GTCGATCAGCACAACCTGGA |