| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044413 |
| Chromosome: | chromosome 17 |
| Location: | 6759233 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746547 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2) | CDS | |
| Cre17.g746597 | (1 of 2) K16296 - serine carboxypeptidase-like clade I [EC:3.4.16.-] (SCPL-I) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTGCATTCTTACGCAACCAGGCGAAGACAGGTGAAGGCGGGTTCCGG |
| Internal bar code: | ACGACTCAAAGTCAAATATCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1449 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAGCAGTGCACAAGGTG |
| Suggested primer 2: | TGGTTGTTACGACCGAGTGG |