Insertion junction: CLIP2.044417_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.044417
Chromosome:chromosome 2
Location:5884116
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g117410 (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCCACGCACCACTCGTCCACGAAGTCGTGCTGCAGGCTGGGCGGCAC
Internal bar code:TTAAAATTTGGTTTAGGAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:881
LEAP-Seq percent confirming:52.1739
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGCCCGGTTAGCTCATT
Suggested primer 2:CCCCCGCTTTTCCAAACATG