| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044424 |
| Chromosome: | chromosome 9 |
| Location: | 2614577 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392251 | (1 of 28) 2.7.7.6 - DNA-directed RNA polymerase / RNA polymerase III | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTTCTGGAGCTTCGAGGTCCGGACCCCGGCCGCGCCCAGCTGCTGAT |
| Internal bar code: | GCTGATTAGATAGGTGGAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1962 |
| LEAP-Seq percent confirming: | 97.8495 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCAGATCCCCAGCCCAT |
| Suggested primer 2: | CCCTACCTGCACCTCTACCT |