Insertion junction: CLIP2.044432_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.044432
Chromosome:chromosome 3
Location:1536440
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g152000 ABA3,MCS1 (1 of 2) K15631 - molybdenum cofactor sulfurtransferase (ABA3); Molybdenum cofactor sulfurase CDS
Cre03.g152050 GOX20,GOX12 Glyoxal oxidase 12; (1 of 2) IPR009880//IPR011043//IPR013783//IPR014756//IPR015202//IPR015916 - Glyoxal oxidase, N-terminal // Galactose oxidase/kelch, beta-propeller // Immunoglobulin-like fold // Immunoglobulin E-set // Domain of unknown function DUF1929 // Galactose oxidase, beta-propeller 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCACCAGCACCGACCCCGCACCAAACCGCCGCCGCAGCCACTCCACA
Internal bar code:TTGACTTTGTAATAGCCTGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCTACGGTATGCAGCTA
Suggested primer 2:TGCCGTAGTACCGTAGTGGA