Insertion junction: CLIP2.044435_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.044435
Chromosome:chromosome 8
Location:3066689
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g375500 ATF1 Glucosamine-fructose-6-phosphate aminotransferase; (1 of 1) 2.6.1.16 - Glutamine--fructose-6-phosphate transaminase (isomerizing) / L-glutamine-D-fructose-6-phosphate amidotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACTCCTCACCCGCGCCCCCGCAGCGACACCATGTACAAGAAGATGGA
Internal bar code:GAGTGGGGCAGCGTGGTCCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:382
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCTAACCCTAAACACCCC
Suggested primer 2:CGCTGGTGGACAAACACATG