| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044443 |
| Chromosome: | chromosome 16 |
| Location: | 1025759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649100 | MAPKKK13 | (1 of 1) K04419 - mitogen-activated protein kinase kinase kinase 11 (MAP3K11, MLK3); Mitogen-Activated Protein Kinase Kinase Kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGACAGCGAGGTCATCCTGGCTGTGGTCACGCGCAAGGCGCGGCCGC |
| Internal bar code: | CCATGAGAGCGCGCGACCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3139 |
| LEAP-Seq percent confirming: | 61.9048 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGGTTCTCCAGTTCGT |
| Suggested primer 2: | TTCCTGCATGAGAACCGCAT |