Insertion junction: CLIP2.044445_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044445
Chromosome:chromosome 17
Location:5544366
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g739550 CPLD12 Conserved in the Plant Lineage and Diatoms; (1 of 11) 2.7.4.3 - Adenylate kinase / Myokinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTTCTGGAGGGCCCCGGGGCCAGTGGGGGCGAGGTGGGGGCGGATGGC
Internal bar code:GCGTCAGTAGTTGGACGTATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2098
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCGAGCATCCAATCTCTC
Suggested primer 2:CAGCGAAACGACGGTTCATC