| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044450 |
| Chromosome: | chromosome 10 |
| Location: | 3166200 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441750 | (1 of 4) PTHR11772//PTHR11772:SF2 - ASPARAGINE SYNTHETASE // ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCGGACGTATGACACTTACCGCCAGGGTAGCAGAACTTTGTGACAGT |
| Internal bar code: | AGCACTGGTATGTATGTGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1874 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACTGTACGCTTTTGCCC |
| Suggested primer 2: | AGGACAGCAGCCGAGTACTA |