| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044466 |
| Chromosome: | chromosome 8 |
| Location: | 627065 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360050 | DUR1 | Urea carboxylase/allophanate hydrolase; (1 of 1) 6.3.4.6 - Urea carboxylase / Urease (ATP-hydrolyzing) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGCGTCACGCACTTCGCGCAGTGCAAGATTGCAATGCCGTCTTCGC |
| Internal bar code: | GTGCGTTCGTTCTGTCTTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1771 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTACCCACACATCCCACA |
| Suggested primer 2: | GCCTGCTATGGACTCTCGTC |